The myeloid cell biomarker EMR1 is ectopically expressed in colon cancer

OBJECTIVE: The microenvironment of colon cancer (CC) is heterogeneous including cells myeloid lineage affecting tumor growth and metastasis. Two functional subtypes have been identified; one (M1) tumor-inhibitory the other (M2) tumor-promoting. Whether three markers EMR1, CD206 CD86 are expressed only in infiltrating or also was investigated. METHODS: Expression investigated CC at mRNA protein levels primary tumors lymph nodes. expression determined 5 cell lines. Protein by two-color immunofluorescence consecutive-sections-immune-staining combined with morphometry using specific antibodies for epithelial CEACAM5 EpCAM. RESULTS: EMR1 CD86, but not CD206, were significantly higher compared to apparently normal tissue (P < 0.0001). both hematoxylin-eosin positive (H&E(+)) H&E(–) nodes patients control = 0.03 P 0.01, respectively). mRNAs 4/5 5/5 lines, respectively, while expressed. Immuno-morphometry revealed that about 20% CD206. Positive as anti-CEACAM5 anti-EpCAM staining. number increased However, colonocytes. Only showed numbers H&E(+) H&E(-) 0.001). correlated CXCL17 expressing cells. CONCLUSION: like chemokine CXCL17, ectopically possibly same